| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310021 |
| CAS号： |
Anti-Akt抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310021Anti-Akt(Phospho-Ser473) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC60
Sensitivity The antibody detects endogenous level of Akt only when phosphorylated at serine 473.
Anti-Akt抗体Immunogen Peptide sequence around phosphorylation site of serine 473 (Q-F-S(p)-Y-S) derived from Human Akt.
Host Rabbit
Clone Number
Antibody type Polyclonal antibody
Anti-Akt抗体Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
Anti-Akt抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using Akt (Phospho-Ser473) Rabbit pAb (310021,1:500 diluted upper) and AKT(pan) Mouse mAb (200323,lower).
Western blot analysis of extracts from SK-BR-3 cells, treated with insulin and EGF, and pretreated with U0126 and LY294002 cells using Akt (Phospho-Ser473) Antibody .
Western blot analysis of extracts from 293 cells, treated with EGF or calf intestinal phosphatase (CIP), using Akt (Phospho-Ser473) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt (Phospho-Ser473) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemical analysis of paraffin- embedded human Lung carcinoma tissue using Akt (Phospho-Ser473) Antibody .
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