| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310023 |
| CAS号： |
Anti-PTEN抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310023Anti-PTEN(Phospho-Ser380/Thr382/Thr383) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC54
Sensitivity The antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383.
Anti-PTEN抗体Immunogen Peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S(p)-D-T(p)-T(p)-D-S) derived from Human PTEN.
Host Rabbit
Clone Number
Antibody type Polyclonal antibody
Purified method
Anti-PTEN抗体Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
Anti-PTEN抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from Hela and C6 cells, treated with EGF or PMA, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Western blot analysis of extracts from Hela cells, treated with IFNa or calf intestinal phosphatase (CIP), using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunofluorescence staining of methanol-fixed MEF cells using PTEN (Phospho-Ser380/Thr382/Thr383) Antibody .
Western blot detection of PTEN(Phospho-Ser380/Thr382/Thr383) in A549 cells untreated or treated with Alkaline phosphatase using PTEN(Phospho-Ser380/Thr382/Thr383) Rabbit pAb (dilution 1:1000, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:54kDa.Observed band size:54kDa.
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