| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310052 |
| CAS号： |
Anti-NFκB-p65抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310052Anti-NFκB-p65(Phospho-Ser529) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC65
Sensitivity The antibody detects endogenous level of NFκB-p65 only when phosphorylated at serine 529.
Anti-NFκB-p65抗体Immunogen Peptide sequence around phosphorylation site of serine 529 (L-L-S(p)-G-D) derived from Human NFκB-p65.
Host Rabbit
Clone Number
Anti-NFκB-p65抗体Antibody type Polyclonal antibody
Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
Anti-NFκB-p65抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from 293 cells untreated or treated with TNF-α using NFκB-p65 (Phospho-Ser529) Antibody .
Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser529) Antibody .
Western blot analysis of extracts from MCF cells, treated with TNFa+CA or calf intestinal phosphatase (CIP), using NFκB-p65 (Phospho-Ser529) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser529) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of NFkB-p65(Phospho-Ser529) in 3T3 and Hela cells untreated or treated with Alkaline phosphatase using NFkB-p65(Phospho-Ser529) Rabbit pAb (dilution 1:500, upper) or β-Actin Mouse mAb (200068-8F10, lower).Predicted band size:65kDa.Observed band size:65kDa.
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