供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL310055 |
CAS号: |
Anti-EGFR抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310055Anti-EGFR(Phospho-Tyr1148) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC175
Sensitivity The antibody detects endogenous level EGFR only when phosphorylated at tyrosine 1172.
Anti-EGFR抗体Immunogen Peptide sequence around phosphorylation site of tyrosine 1172 (P-D-Y(p)-Q-Q) derived from Human EGFR.
Host Rabbit
Clone Number
Antibody type Polyclonal antibody
Purified method
Anti-EGFR抗体Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
Anti-EGFR抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using EGFR (Phospho-Tyr1148) Rabbit pAb (310055,1:500 diluted,upper) and EGF Receptor Mouse mAb (201012-3F12,lower).
Western blot analysis of extracts from A431 cells untreated or treated with EGF using EGFR (Phospho-Tyr1172) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR (Phospho-Tyr1172) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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