供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL310060 |
CAS号: |
Anti-eIF4E抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310060Anti-eIF4E(Phospho-Ser209) Rabbit pAbRabbit pAbHumanWB,IHC,ICC25
Sensitivity The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Anti-eIF4E抗体Immunogen Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.
Host Rabbit
Clone Number
Antibody type Polyclonal antibody
Anti-eIF4E抗体Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
Anti-eIF4E抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from Hela cells, untreated or treated with UV, using eIF4E (Phospho-Ser209) Rabbit pAb (310060,1:500 diluted,upper) or Beta actin Mouse mAb (200068-8F10,lower).
Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) Antibody .
Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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