Anti-eIF4E抗体

参考价:¥1190
供货周期: 现货
品牌: 康朗生物
规格: 100ul/50ul/25ul
货号: KL310060
CAS号:
上海康朗生物科技有限公司
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产品详情

Anti-eIF4E抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

310060Anti-eIF4E(Phospho-Ser209) Rabbit pAbRabbit pAbHumanWB,IHC,ICC25

Sensitivity  The antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.


Anti-eIF4E抗体Immunogen  Peptide sequence around phosphorylation site of serine 209 (S-G-S(p)-T-T) derived from Human eIF4E.


Host  Rabbit


Clone Number  


Antibody type  Polyclonal antibody


Anti-eIF4E抗体Purified method  


Isotype  IgG


Formulation  Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.


Dilution

WB~~1:500-1:1000

ICC~~1:100-1:200

Anti-eIF4E抗体IHC~~1:50-1:100

Application Images

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Western blot analysis of extracts from Hela cells, untreated or treated with UV, using eIF4E (Phospho-Ser209) Rabbit pAb (310060,1:500 diluted,upper) or Beta actin Mouse mAb (200068-8F10,lower).


Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209) Antibody .


Western blot analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody .


Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209) Antibody (left) or the same antibody preincubated with blocking peptide (right).


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