| 供货周期： | 现货 |
| 品牌： | 康朗生物 |
| 规格： | 100ul/50ul/25ul |
| 货号： | KL310081 |
| CAS号： |
Anti-Akt抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310081Anti-Akt(Phospho-Thr450) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC60
Sensitivity The antibody detects endogenous level of AKT1 only when phosphorylated at threonine 450.
Anti-Akt抗体Immunogen Peptide sequence around phosphorylation site of threonine 450 (T-I-T(p)-P-P) derived from Human AKT1.
Host Rabbit
Clone Number
Antibody type Polyclonal antibody
Anti-Akt抗体Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
Anti-Akt抗体IHC~~1:50-1:100
Application Images-expand |
Western blot analysis of extracts from A549 cells, untreated or treated with EGF(10ng/ml,10min), using AKT (phospho-Thr450) Rabbit pAb (310081,1:500 diluted,upper) and AKT(pan) Mouse mAb (200323,lower).
Immunofluorescence staining of methanol-fixed Hela cells using AKT1 (phospho-Thr450) Antibody .
Western blot analysis of extracts from 293 cells untreated or treated with EGF using AKT1 (phospho-Thr450) Antibody .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1 (Phospho-Thr450) Antibody (left) or the same antibody preincubated with blocking peptide (right).
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