供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL612226 |
CAS号: |
Anti-MCL1抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
612226Anti-MCL1 (BH3 Domain Specific) Rabbit pAbRabbit pAbHuman,Mouse,RatWB, FC, IHC-P
Sensitivity
Anti-MCL1抗体Immunogen This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-226 amino acids from human MCL1.
Host Rabbit
Clone Number RB00961
Anti-MCL1抗体Antibody type Polyclonal antibody
Purified method Affinity purified
Isotype Rabbit Ig
Formulation Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Anti-MCL1抗体Dilution
WB~~1:2000
IHC~~1:10~50
FCM~~1:10~50
Application Images-expand |
Western blot analysis of extracts from Raji,Ramos and Molt-4 cell lysates using MCL1 Rabbit pAb (1:500 diluted).Predicted band size:37KDa.Observed band size:37KDa.
Formalin-fixed and paraffin-embedded human breast carcinoma with MCL1 Antibody (BH3 Domain Specific), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemi
Flow cytometric analysis of ZR-75-1 cells using MCL1 Antibody (BH3 Domain Specific)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes : Anti-MCL1 Antibody (BH3 Domain Specific) at 1:2000 dilution Lane 1: Raji whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: A431 whole cell lysate Lane 4: F9 whole cell lysate Lane 5: rat heart lysate Lysates/proteins at 20 μg per lane
Western blot analysis of anti-Mcl-1 BH3 Domain Pab (Cat. #612226) in Ramos cell line lysates (35ug/lane). Mcl-1-BH3(arrow) was detected using the purified Pab.
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