Anti-EN1抗体

Anti-EN1抗体

Product Properties

Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)

616140Anti-EN1(Engrailed 1) (N-term) Rabbit pAbRabbit pAbHumanWB, IF, IHC-P

Sensitivity  

Anti-EN1抗体Immunogen  This EN1 (Engrailed 1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EN1 (Engrailed 1).

Host  Rabbit

Clone Number  RB13885

Antibody type  Polyclonal antibody

Anti-EN1抗体Purified method  Affinity purified

Isotype  Rabbit Ig

Formulation  Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Dilution

WB~~1:1000

Anti-EN1抗体ICC~~1:25

IHC~~1:25

All lanes : Anti-EN1 Antibody (N-term) at 1:1000 dilution Lane 1: human fetal brain lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS (Human Sarcoma cell line) cells labeling Pdx1 with 616140 at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary

616140 staining EN1 in Human tonsil tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval wa

All lanes : Anti-EN1 Antibody (N-term) at 1:2000 dilution Lane 1: NCI-H460 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lane 3: PC-3 whole cell lysates Lane 4: Jurkat whole cell lysates Lane 5: 293T whole cell lysates Lysates/proteins at 20 μg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 40 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of lysates from 293, SH-SY5Y, NCI-H460 cell line (from left to right), using EN1 Antibody (N-term)(Cat. #616140). 616140 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

Fluorescent image of U251 cell stained with EN1 Antibody (N-term)(Cat#616140).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EN1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).EN1 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#616140), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for im