供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL310022 |
CAS号: |
Anti-Akt抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
310022Anti-Akt(Phospho-Thr308) Rabbit pAbRabbit pAbHuman,Mouse,RatWB,IHC,ICC60
Sensitivity The antibody detects endogenous level of Akt only when phosphorylated at threonine 308.
Anti-Akt抗体Immunogen Peptide sequence around phosphorylation site of threonine 308 (M-K-T(p)-F-C) derived from Human Akt.
Host Rabbit
Clone Number
Anti-Akt抗体Antibody type Polyclonal antibody
Purified method
Isotype IgG
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Anti-Akt抗体Dilution
WB~~1:500-1:1000
ICC~~1:100-1:200
IHC~~1:50-1:100
Application Images-expand |
Western blot detection of Akt (Phospho-Thr308) in Hela cells untreated or serum-treated, using Akt (Phospho-Thr308) Rabbit pAb (dilution 1:1000, upper) or Akt(pan) Mouse mAb (200323, lower).Predicted band size:60kDa.Observed band size:60kDa.
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody .
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody (left) or the same antibody preincubated with blocking peptide #51055 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing nuclear dot staining using Akt (Phospho-Thr308) Antibody .
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using Akt (Phospho-Thr308) Antibody .
Western blot analysis of extracts from Hela cells untreated or treated with EGF, serum or both using Akt (Phospho-Thr308) Antibody .
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