供货周期: | 现货 |
品牌: | 康朗生物 |
规格: | 100ul/50ul/25ul |
货号: | KL618122 |
CAS号: |
Anti-B-Raf 抗体
Product Properties
Cat CodeProduct NameClonalityCross reactivityApplicationMW(kDa)
618122Anti-B-Raf Mouse mAbMouse mAbHuman,Mouse,Rat.WB, IF86kDa
Sensitivity
Anti-B-Raf 抗体Immunogen This BRAF Monoclonal antibody is generated from mouse immunized with BRAF recombinant protein.
Host Mouse
Clone Number 1F1-F7-A8
Anti-B-Raf 抗体Antibody type Monoclonal antibody
Purified method Affinity purified
Isotype IgG1κ
Formulation Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Anti-B-Raf 抗体Dilution
WB~~1:1000
ICC~~1:10~50
Application Images-expand |
Western blot detection of B-Raf in A549,MCF7,3T3,C6 and Hela cell lysates using B-Raf mouse mAb (1:5000 diluted).Predicted band size:84KDa.Observed band size:86KDa.
Fluorescent image of C2C12 cell stained with BRAF Antibody(Cat#618122/SG100521B).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody (1:25, 1 h at 37℃. For secondary anti
Western blot analysis of anti-BRAF Antibody in HL-60 cell line lysates (35μg/lane). BRAF (arrow) was detected using the purified Mab.
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