ProPac IMAC蛋白和肽柱 063667_价格_上海希言科学仪器有限公司

产品详情

ProPac IMAC Protein and Peptide Column

Analytical Column for Immobilized Metal Affinity Chromatography

The ProPac^® IMAC-10 is an analytical HPLC column packed with 10 μm, nonporous, polymeric beads that are coated with a hydrophilic layer, then grafted with poly(IDA) chains. The poly(IDA) grafts are converted to metal-containing nanoparticles when the column is charged with metal. It is these nanoparticles that act as IMAC interaction sites for individual proteins and provide the ProPac IMAC-10 with its high resolving power.

State-of-the-art technology for reusable columns with metal tailored specificity
Resolve target proteins using a single column in a high resolution gradient run
Retention control by imidazole concentration or pH gradient
High purity separations of metal-binding proteins
Wide range of metal-specific applications

The ProPac IMAC-10 design maximizes the number of sterically accessible ligands for binding to proteins, ensuring that the proteins remain tightly bound during separation. It can be operated between pH 2–12 and is compatible with commonly used protein purification reagents (non-ionic detergents, reducing agents). It can be used under native or denaturing HPLC conditions

The ProPac IMAC-10 is shipped free from any metal and ready to be charged with the metal of your choice. To avoid contact of the metal solution with your system, charge the IMAC-10 with a loading column (an empty column body you fill with your metal solution, then place between the pump and the IMAC-10 column).

ProPac IMAC Columns Specifications
Maximum Operating Pressure	<3000 psi (21 MPa)
Temperature Range	<60^oC Recommended Temperature: Controlled ambient
Typical Operating Conditions	<600 psi at 1.0 mL/min (4 × 250mm) Recommended flow rate: 1.0 mL/min
Mobile Phase Compatibility	pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Resin Compostion	10 μm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts.
Metal Ion Capacity (μmol Cu)	>80 mmol Cu (4 × 250 mm analytical column)
Chelating Metals	Ni, Fe, Cu, others
Analytical Column Dimensions	1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm
Loading Column Dimensions	4 × 50 mm, 9 × 50 mm, 9 × 250 mm

Analytical Columns
ProPac IMAC-10 Column (1 x 50 mm)	063617
ProPac IMAC-10 Column (2 x 50 mm)	063272
ProPac IMAC-10 Column (4 x 50 mm)	063276
ProPac IMAC-10 Column (9 x 50 mm)	063615
ProPac IMAC-10 Column (2 x 250 mm)	063274
ProPac IMAC-10 Column (4 x 250 mm)	063278
ProPac IMAC-10 Column (9 x 250 mm)	063280
ProPac IMAC-10 Column (22 x 250 mm)	063282

Accessories
IMAC Loading Column (4 x 50 mm)	063667
IMAC Loading Column (9 x 50 mm)	063710
IMAC Loading Column (9 x 250 mm)	063718

ProPac IMAC蛋白和肽柱 063667信息由上海希言科学仪器有限公司为您提供，如您想了解更多关于ProPac IMAC蛋白和肽柱 063667报价、型号、参数等信息，欢迎来电或留言咨询。除供应ProPac IMAC蛋白和肽柱 063667外，上海希言科学仪器有限公司还可为您提供其他等产品，公司有专业的客户服务团队，是您值得信赖的合作伙伴。

供货周期：	现货
品牌：	戴安
货号：	63667