PepSwift系列色谱柱 164542_价格_上海希言科学仪器有限公司

产品详情

PepSwift Columns

Monolithic Columns for Fast, High-Resolution Protein and Peptide Analysis

PepSwift monolithic columns are specially designed for fast separation and identification of proteins and peptides using nano and capillary LC coupled to MS. PepSwift columns are based on a polystyrene divinylbenzene copolymer bed. The bed structure of the column offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages for high-sensitivity proteomics applications.

Polymeric monolithic stationary phases
High-speed protein/peptide separations (< 10 min)
High separation efficiency (> 200.000 plates/m)
High column-to-column reproducibility
High sensitivity in LC/MS
Superior lifetime
Available in 100 μm, 200 μm and 500 μm i.d
Available in 5 and 25 cm length (only 100 and 200 μm i.d.)
nanoViper fitting

PepSwift Trap columns can be used for preconcentration and desalting of samples consisting of peptides and proteins without negative impact on the chromatographic performance or recovery of the compounds. Various ion-pairing agents can be used in the loading solvent and/or mobile phases to change the selectivity of the separation or improve the trapping efficiency.

PepSwift and ProSwift Capillary Monolithic Reversed-Phase Columns Specifications
	PepSwift RP
Typical Applications	Peptides, proteins, and oligonucleotides
Column Dimensions	100 μm × 50 mm	200 μm × 50 mm	500 μm × 50 mm
Base Material	Polystyrene/divinyl benzene	Polystyrene/divinyl benzene	Polystyrene/divinyl benzene
Chemistry	Phenyl	Phenyl	Phenyl
Recommended Flow Rate	700–1000 nL/min	2–3 μL/min	15–25 μL/min
Temperature	80 ?C	80 ?C	80 ?C
Maximum Pressure	4350 psi (30 MPa)	4350 psi (30 MPa)	4350 psi (30 MPa)
Recommended Sample Quantity	5 ng*	20 ng*	0.12 μg*
Sample Capacity***	0.2 ng	0.6 ng	3.8 ng
pH Stability	1–10	1–10	1–10
Solvent Compatibility	Most common RP solvents	Most common RP solvents	Most common RP solvents

*Tryptic digest of six proteins
**E. coli proteins
*** Dynamic loading capacity is defined as the 10% increase in peak width measured at half height induced by injected mass of a single peptide (tryptic peptide of cytochrome c for PepSwift RP) and intact protein (cytochrome c for ProSwift RP-10R), respectively

PepSwift and ProSwift Monolith Columns
PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper	164584
PepSwift Monolithic Nano Column, 100 μm i.d. × 5 cm, nanoViper	164584
PepSwift Monolithic Nano Column, 100 μm i.d. x 25 cm, nanoViper	164543
PepSwift Monolithic Capillary Column, 200 μm i.d. × 5 cm	161409
PepSwift Monolithic Capillary Column, 200 μm i.d. x 5 cm, nanoViper	164557
PepSwift Monolithic Capillary Column, 200 μm i.d. x 25 cm, nanoViper	164542
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm	164087
PepSwift Monolithic Capillary Column, 500 μm i.d. × 5 cm, nanoViper	164585
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper	164586
ProSwift RP-10R Monolithic Capillary Column, 1 mm i.d. × 5 cm, nanoViper	164586

PepSwift Trap Columns
PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper	164558
PepSwift Monolithic Trap Column, 200 μm x 5 mm, (set of 2), nanoViper	164558

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供货周期：	现货
品牌：	戴安
货号：	164542