MAbPac蛋白A亲和柱 082539_价格_上海希言科学仪器有限公司

产品详情

MAbPac Protein A Affinty Column

MAbPac Protein A Column for Monoclonal Antibody Titer Analysis

The Thermo Scientific™ MAbPac™ Protein A column is a high-performance, affinity chromatography column specifically designed for fast, accurate determination of monoclonal antibody (MAb) concentration in the harvest cell culture (HCC). Early in the development of recombinant MAbs, a large of number of HCC samples must be screened for IgG titer. Affinity chromatography employing a Protein A ligand is often used to determine the MAb concentration. The MAbPac Protein A column is a high-pressure liquid chromatography (HPLC) column designed to provide fast, accurate, titer analysis with linearity over a wide concentration range without carryover.

The MAbPac Protein A column is based on a novel nonporous polymeric resin consisting of a hydrophobic divinylbenzene core and a hydrophilic surface. This proprietary resin is designed for fast mass transfer, resulting in efficient separations without carryover. The recombinant Protein A ligands are covalently attached onto the hydrophilic resin surface through their amine groups to provide a very rugged, reproducible column. The column is available in a HPLC bioinert, 4 × 35 mm PEEK column body.

Product Highlights:

? A novel nonporous polymeric resin with a hydrophilic surface
? Functionalized with the recombinant protein A ligands
? Wide range of MAb quantitation: 0.025–5 mg/mL
? Fast analysis: 2 min/analysis
? Rugged
? Low pressure: column pressure is less than 250 psi at 2 mL/min

The MAbPac Protein A column can be used for both titer analysis or concentrating and purifying IgG material for later experiments or two-dimensional (2D) chromatography.

MAbPac Protein A Column Specifications
Column Dimension	4 × 35 mm
Base Matrix Material	Hydrophilic nonporous resin
Surface Ligand	Protein A
Particle Size	12 μm
Binding capacity	~3.5 mg IgG/g resin
Dynamic Loading Capacity	≤100 μg IgG/column at 2 mL/min flow rate
pH Range	1.9–7.5
Maximum Flow rate	Up to 2 mL/min
Maximum Pressure	≤1000 psi
Operating Temperature	<30 ?C

Analytical Columns
MAbPac Protein A, 12 μm, Analytical Column (4 x 35mm)	082539

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供货周期：	现货
品牌：	戴安
货号：	82539