去整合素样金属蛋白酶2抗体

去整合素样金属蛋白酶2抗体

英文名称    ADAM2    

中文名称    去整合素样金属蛋白酶2抗体    

别    名    A disintegrin and metalloproteinase domain 2; ADAM 2; ADAM metallopeptidase domain 2; ADAM2_MOUSE; AI323749; Cancer/testis antigen 15; CRYN1; CRYN2; CT15; Disintegrin and metalloproteinase domain-containing protein 2; Fertilin beta; Fertilin subunit beta; Ftnb; PH 30; PH 30b; PH-30; PH30; Ph30 beta; PH30-beta.      

研究领域    肿瘤  细胞生物  免疫学  信号转导  激酶和磷酸酶  细胞骨架  细胞外基质  泛素      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    63/80kDa    

细胞定位    细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthetic peptide derived from mouse ADAM2:451-550/735 <Extracellular>    

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    ADAM-2, also known as PH-30?and Fertilin-? was first described as a sperm-egg fusion protein from guinea pig. A member of the metalloproteinase family containing disintigrin-like domains (ADAMs), the function of ADAM-2 is still poorly understood. Unlike ADAM-1, ADAM-2 does not contain the canonical HExxHxxxxxH zinc metalloproteinase motif, and is not thought to be proteolytically active. Like the other ADAMs, ADAM-2 domain structure consists of a signal sequence followed by a propeptide domain, a metalloproteinase domain, a disintegrin domain cysteine-rich domain, an EGF-like domain, a transmembrane domain, and a cytoplasmic domain. Three isoforms of ADAM-2 are reported to date, which differ in the beginning of the metalloproteinase domain and cysteine-rich domain. The sequences are coded from intronless genes. The longest ADAM-2 message encodes a protein of 735 amino acids, with a predicted mass of 82.5 kDa and a pI of 5.73. The 716 amino acid form of ADAM-2 has a deletion in the start of the metalloproteinase domain, relative to the longer form, and has a predicted mass of 80.2 kDa and pI of 5.75. The shorter form of 579 amino acids shared the deletion in the MP domain, and also has a deletion in the cystein-rich domain. Localized on the surface of sperm, the ADAM-2 isoforms are thought to form a heterodimer with ADAM-1 (fertilin-a), and facilitate sperm-egg fusion, although there is some controversy about the precise actions the proteins play. Integrin a6? on the egg surface is thought to dock with a QDECD motif in the disintegrin domain of ADAM-2, and there is some speculation that ADAM-1/ADAM-2 heterodimewr initiated ADAM-3 production on the cell surface.