磷酸化富含脯氨酸的酪氨酸激酶2抗体

磷酸化富含脯氨酸的酪氨酸激酶2抗体

英文名称    phospho-PYK2 (Tyr402)    

中文名称    磷酸化富含脯氨酸的酪氨酸激酶2抗体    

别    名    p-PTK2B(Tyr402); Pyk2(Phospho-Tyr402); PTK2B(phospho Y402); CADTK; CAK beta; CAKB antibody; CAKbeta; Calcium dependent tyrosine kinase; Calcium-dependent tyrosine kinase; Cell adhesion kinase beta; E430023O05Rik; EC 2.7.10.2; FADK 2; FADK2; FAK 2; FAK1; FAK2; Focal Adhesion Kinase 2; MGC124628; PKB; pp125FAK; Proline Rich Tyrosine Kinase 2; Proline-rich tyrosine kinase 2; Protein kinase B; Protein Tyrosine Kinase 2 Beta; PTK 2B; PTK antibody PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK 2; PYK2; RAFTK; Related adhesion focal tyrosine kinase; FAK2_HUMAN; PTK2B protein tyrosine kinase 2 beta.      

产品类型    磷酸化抗体     

研究领域    肿瘤  免疫学  细胞凋亡  转录调节因子  激酶和磷酸酶  细胞粘附分子      

抗体来源    Rabbit    

克隆类型    Polyclonal    

交叉反应    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,     

产品应用    WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    116kDa    

细胞定位    细胞核 细胞浆 细胞膜     

性    状    Lyophilized or Liquid    

浓    度    1mg/ml    

免 疫 原    KLH conjugated synthesised phosphopeptide derived from human Pyk2/PTK2B around the phosphorylation site of Tyr402:DI(p-Y)AE     

亚    型    IgG    

纯化方法    affinity purified by Protein A    

储 存 液    0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.    

保存条件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

PubMed    PubMed    

产品介绍    This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

富含脯氨酸的酪氨酸激酶 2 (protein richtyro sinekinase 2 ,PYK2 ) ,又称细胞粘附激酶β(cellularadhesionkinaseβ ,CAKβ)、相关粘附聚焦酪氨酸激酶(relatedadhesionfocaltyrosinekinase ,RAFTK) ,是粘着斑激酶 (focaladhesionkinase,FAK)家族的成员之一。PYK2是FAK家族的成员之一 ,是一种钙依赖性酪氨酸激酶 ,在氨基酸序列上与FAK有 4 5 %的同源性。它的活化涉及了多条信号传导通路 ,与离子通道的调节、细胞骨架的联系及细胞增殖、凋亡密切相关。血管紧张素Ⅱ、一氧化氮可调节PYK2的活性。在神经系统中高度表达的蛋白酪氨酸激酶 （Pyk2），其酪氨酸残基因为各种G蛋白偶联受体和胞外信号而被磷酸化，从而增加胞内钙离子的浓度.