供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 50μl |
货号: | ZY-0407R |
CAS号: |
Anti-Cytokeratin 17 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to near C-terminal residues of mouse CK-17.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cytoplasm.
数据链接SwissProt: Q04695 Human
SwissProt: Q9QWL7 Mouse
SwissProt: Q6IFU8 Rat
其它名称
WB:1:2,000
ICC:1:50-1:200
IHC-P:1:50-1:200
FC:1:50-1:100
Fig1: Western blot analysis of Cytokeratin 17 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Cytokeratin 17 in A431 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-4) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig3: ICC staining Cytokeratin 17 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-4) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: ICC staining Cytokeratin 17 in SK-Br-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-4) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-4) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Cytokeratin 17 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (0407-4) at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Fig7: Flow cytometric analysis of Cytokeratin 17 was done on Hela cells. The cells were fixed, permeabilized and stained with Cytokeratin 17 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antib
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