供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 50μl |
货号: | ZY-1912R |
CAS号: |
Anti-LOX 1 antibody
种属反应性Human,Mouse,Rat,Cow,Rabbit
验证应用WB,IHC-P,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from rabbit LOX-1 201-273/273
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Cell membrane; Single-pass type II membrane protein. Secreted. Note=A secreted form also exists.
数据链接SwissProt: Q9XTA8 Human
其它名称
WB:1:500-2000
IHC-P:1:400-800
FC:1μg /test
Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- LOX1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kD
Observed band size: 50 kD
Fig2: Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti-LOX 1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31/50 kD
Observed band size: 50 kD
Fig3: Positive control: A549 cells
Concebtration: 1μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
Fig4: Blank control: RSC96 (blue).
Primary Antibody:Rabbit Anti- LOX 1 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig6: Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated (ER1912-42) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
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