供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY-6803-41R |
CAS号: |
Anti-LOXL2 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Recombinant protein within human LOXL2 aa 300-480.
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein affinity purified.
亚细胞定位Endoplasmic reticulum. Nucleus. Secreted.
其它名称
WB: 1:500-1:1,000
ICC: 1:100
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of LOXL2 on A549 and A431 cell lysate using anti-LOXL2 antibody at 1/500 dilution.
Fig2: ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig8: Flow cytometric analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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