供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6907-11R |
CAS号: |
Anti-Cytochrome B antibody
种属反应性Human,Mouse,Rat,Pig
验证应用WB,FC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Cytochrome B 101-200/388
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型IgG
纯化方式affinity purified by Protein A
亚细胞定位Mitochondrion inner membrane; Multi-pass membrane protein.
其它名称
WB:1:500-2000
FC:1ug/test
Fig1: U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Cytochrome B Antibodyat 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Fig2: Sample:
Hela(Human) Cell Lysate at 30 ug
Primary: Anti-Cytochrome B at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
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