供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6512-15R |
CAS号: |
Anti-GPC1 antibody
种属反应性Human
验证应用WB,ICC,IHC-P,FC
抗体类型兔多抗
免疫原Peptide
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃after thawing. Aliquot store at -20℃or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Membrane. Secreted.
其它名称
FLJ38078 antibody
glypican antibody
Glypican proteoglycan 1 antibody
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of Glypican-1 on different cell lysate using anti- Glypican-1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: SK-Br-3
Fig2: ICC staining Glypican-1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Glypican-1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Glypican-1 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Glypican-1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of MCF-7 cells with Glypican-1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
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