供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6919-24R |
CAS号: |
Anti-PABP antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC/IF
抗体类型兔多抗
免疫原Synthetic peptide within Human PABP aa 101-200.
偶联Non-conjugated
形态Liquid
浓度1mg/ml
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Protein A purified.
亚细胞定位Cytoplasm, Nucleus
其它名称
PAB 1 antibody
PAB1 antibody
PABP 1 antibody
WB:1:500-2000
IHC-P:1:500-1000
ICC/IF:1:100-500
Fig1: Western blot analysis of PABP on NIH/3T3 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PABP antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-PABP antibody
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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