供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6919-22R |
CAS号: |
Anti-TNF alpha antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human TNF-alpha:181-235/235
偶联Non-conjugated
形态Liquid
浓度1mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Cell membrane, Secreted.
其它名称
APC1 antibody
APC1 protein antibody
Cachectin antibody
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
Fig1: Western blot analysis of TNF alpha against mouse liver tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of TNF alpha against mouse Diseased lung tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
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