供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6919-20R |
CAS号: |
Anti-Osteocalcin antibody
种属反应性Human, Mouse, Rat, Dog, Pig, Cow, Horse
验证应用WB, IHC-P, ICC
抗体类型兔多抗
免疫原KLH conjugated synthetic peptide derived from human Osteocalcin:21-100/100
偶联Non-conjugated
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 1%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Affinity purified by Protein A
亚细胞定位Secreted.
其它名称
BGLAP antibody
BGP antibody
Bone gamma carboxyglutamate (gla) protein antibody
WB: 1:500-1:2000
IHC-P: 1:50-1:200
ICC: 1:50-1:200
Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (ER1919-20) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (ER1919-20) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at [1:500] for 40 minutes and DAPI staining of the nu Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium clei.
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