供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6001-57R |
CAS号: |
Anti-Nucleoporin p62 antibody
种属反应性Human
验证应用WB,IHC
抗体类型兔多抗
免疫原Synthetic peptide within human Nucleoporin p62 aa 470-522.
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cytoplasm, Cytoskeleton, Nuclear pore complex, Nucleus.
其它名称
62 kDa nucleoporin antibody
DKFZp547L134 antibody
FLJ20822 antibody
WB: 1:500-1:1,000
IHC: 1:100-1:400
Fig1: Western blot analysis of Nucleoporin p62 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: K562 cell lysate
Lane 2: A431 cell lysate
Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Nucleoporin p62 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human rectum tissue using anti-Nucleoporin p62 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!
相关产品
彩色预染蛋白Marker(10-180 kDa,三色)
Super GelBlueTM 核酸染料, 10,000× in water
彩色预染蛋白Marker(10-180 kDa,三色)
彩色预染蛋白Marker(10-250kDa, 双色)
琼脂糖 A2015
Super ECL Plus(超敏化学发光检测试剂盒)
PAGE 彩色快速凝胶制备试剂盒(10%)
Minerva Super Fusion Cloning Kit(无缝克隆试剂盒)
Cell Counting Kit-8(CCK-8)细胞增殖检测试剂盒
FITC-Annexin V/PI 细胞凋亡试剂盒
呋喃西林快速检测卡
呋喃妥因快速检测卡
呋喃它酮快速检测试剂盒
呋喃妥因快速检测试剂盒
呋喃西林快速检测试剂盒
关注
拨打电话
留言咨询