供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 兔多抗 |
货号: | ZY6512-3R |
CAS号: |
Anti-GPCR RDC1/CXCR-7 antibody
种属反应性Human
验证应用WB,ICC,FC
抗体类型兔多抗
免疫原Synthetic peptide within C terminal human CXCR-7.
偶联Non-conjugated
形态Liquid
浓度1 mg/ml.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified.
亚细胞定位Cell membrane, Cytoplasm, Endosome, Membrane
其它名称
ACKR3 antibody
atypical chemokine receptor 3 antibody
C X C chemokine receptor type 7 antibody
WB: 1:500-1:1,000
ICC: 1:50-1:200
FC: 1:50-1:100
Fig1: Western blot analysis of GPCR RDC1/CXCR-7 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Jurkat cell lysate
Fig2: ICC staining of GPCR RDC1/CXCR-7 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining of GPCR RDC1/CXCR-7 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: Flow cytometric analysis of GPCR RDC1/CXCR-7 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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