供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 |
货号: | ZY640707M |
CAS号: |
Anti-GFAP antibody
种属反应性Human,Mouse,Rat
验证应用WB,IHC-P,ICC,FC
抗体类型小鼠单抗
免疫原peptide
偶联Non-conjugated
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein A purified.
亚细胞定位Cytoplasm, intermediate filament
其它名称
wu:fb34h11 antibody
ALXDRD antibody
cb345 antibody
WB: 1:2,000-1:5,000
ICC:1:200
IHC-P: 1:200
FC: 1:200
Fig1: Western blot analysis of GFAP on different cell lysates using anti- GFAP antibody at 1/5000 dilution.
Positive control: Line 1: Rat brain Line2 :Human brain
Fig2: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GFAP antibody. Counter stained with hematoxylin.
Fig3: ICC staining of GFAP in A172 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining of GFAP in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Hela cells with GFAP antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
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