供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 |
货号: | ZY6704M |
CAS号: |
Anti-Sequestosome-1 antibody
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P,FC
抗体类型小鼠单抗
免疫原Synthetic peptide (KLH-coupled) within human SQSTM1 40-100 aa.
偶联Non-conjugated
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Peptide affinity purified.
亚细胞定位Cytoplasm, Nucleus
其它名称
A170 antibody
DMRV antibody
EBI 3 associated protein of 60 kDa antibody
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:200
FC: 1 μg per 1 x 106 cells
Fig1: Western blot analysis of SQSTM1 on different cell lysates using anti-SQSTM1 antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Fig2: ICC staining SQSTM1 in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti- SQSTM1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-SQSTM1 antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of Hela cells with SQSTM1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.
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