Anti-CD87 antibody

Anti-CD87 antibody

• 种属反应性Human

• 验证应用WB,FC

• 抗体类型小鼠单抗

• 免疫原Purified recombinant fragment of human CD87 (AA: 23-305) expressed in E. Coli.

• 偶联Non-conjugated

• Anti-CD87 antibody性能

• 形态Liquid

• 浓度1 mg/mL

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS with 0.05% sodium azide.

• 亚型IgG2b

• 纯化方式Protein G purified.

• 亚细胞定位Secreted and Cell membrane.

• 其它名称

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• CD 87 antibody

• CD87 antibody

• CD87 antigen antibody

• Anti-CD87 antibody应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

• 

  Fig1: Western blot analysis of CD87 against human CD87 (AA: 23-305) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of against HEK293 (1) and CD87 (AA: 23-305)-hIgGFc transfected HEK293 (2) cell lysate.Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig3: Flow cytometric analysis of CD87 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody(green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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