供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY6901-05M |
CAS号: |
Anti-Auxin response factor antibody
种属反应性Camellia sinensis
验证应用WB,IHC-P
抗体类型小鼠单抗
免疫原Recombinant protein within Camellia sinensis Auxin response factor aa 401-586.
偶联Non-conjugated
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein affinity purified.
亚细胞定位Nucleus.
WB:1:500-1:1,000
IHC-P:1:50-1:200
Fig1: Western blot analysis of Auxin response factor on tea leafbud lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunohistochemical analysis of paraffin-embedded leafbud using anti-Auxin response factor antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
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