供货周期: | 现货 |
品牌: | 泽叶生物 |
规格: | 小鼠单抗 50 μl |
货号: | ZY6901-97M |
CAS号: |
Anti-Cytokeratin 20 antibody
种属反应性Human
验证应用IHC-P
抗体类型小鼠单抗
免疫原Synthetic peptide within N-terminal Human Cytokeratin 20.
偶联Non-conjugated
形态Liquid
浓度2 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG1
纯化方式Protein G affinity purified.
亚细胞定位Cytoplasm.
其它名称
CK 20 antibody
CK-20 antibody
CK20 antibody
IHC-P:1:50-1:200
Fig1: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig2: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Cytokeratin 20 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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