供货周期: | 现货 |
品牌: | 百欧博伟生物 |
规格: | 1ml甘油管 赠送一个平板 |
货号: | bio-82268 |
CAS号: |
E.coli MG1655 百欧博伟质粒载体
产品信息:
平台编号:bio-82268
规格:1ml甘油管 赠送一个平板
英文名称:E.coli MG1655
中文名称:大肠杆菌MG1655
货期:款到1-3天出库
运输:冰袋泡沫盒快递
培养方式:LB,37℃
保存:1ml甘油管,-80℃
注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
基因型:
F- lambda- ilvG- rfb-50 rph-1(NC_000913)
基本信息:
抗性:无
培养基:LB
菌株类别:大肠杆菌
培养条件:37℃,有氧,LB
质粒转化:42℃热激
保存方式:20%甘油,-20℃
基本应用:用于基因克隆
备注:大肠杆菌K12系MG1655为非致病性菌株,该菌株基因组大小为4639221bp。
菌株简介:
K-12 MG1655 是一株 K 系列的大肠杆菌,经检测无 Amp 和 Kan 抗性,可用 LB 培养基,37℃培养,其代谢类型是异养兼性厌氧型,可用于代谢研究、基因编辑等实验。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that thestrain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994). MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's labfrom a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from astool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB andM9 minimal medium (+ Glucose + 1ug/ml thiamine).
注意事项:
1、为了您的安全和健康,请穿实验服并戴一次性手套操作。本产品仅可用于实验室研究,不能用于动物,人体以及作为食品添加剂等用途。
2、使用甘油菌时可不完全融解,在甘油菌表面蘸取少量涂布固体琼脂平板即可,也可完全融解后使用,但随着冻融次数的增加,菌株的活力会逐渐下降。
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