供货周期: | 现货 |
品牌: | 百欧博伟生物 |
规格: | freeze-dried |
货号: | bio-72861 |
CAS号: |
BB152 坎氏弧菌 百欧博伟生物 微生物菌种
坎氏弧菌是Vibrio属的微生物,原产地为中国。
一、菌种简介
平台编号:bio-72861
规格:freeze-dried
拉丁属名:Vibrio campbellii
中文名:坎氏弧菌
属:Vibrio
原产地:中国
模式菌株:非模式菌株
注意事项:仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用
二、产品概述
Vibrio campbellii (Baumann et al.) Baumann et al. 拉丁名
(ATCC? BAA-1119?) 统一编号
Deposited As Vibrio harveyi (Johnson and Shunk) Baumann et al.
Strain Designations 菌株别名: BB152 /
Type Strain 模式菌株 : no /
Biosafety Level 生物安全等级: 1
Application 用途 Quorum sensing bacterium Isolation Sensor mutant derived from Vibrio harveyi strain BB120, New Jersey, United States, 1993
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式freeze-dried
Storage Conditions 保藏方法
Frozen (冻干物) : -80℃ or colder
Freeze-Dried(冷冻物): 2℃ to 8℃
Live Culture: See Propagation Section
Preceptrol? no
Genotype luxM::tn5Kan
Type Strain 模式菌株 no
Comments 注释 This is one of several strains (ATCC BAA-1116 through ATCC BAA-1121) included in a "quorum sensing reporter kit" developed by the depositor. Mutant sensor strain that responds only to AI-2 autoinducer Vibrio harveyi BAA-1116 (and its derived mutants in panel MP-6) has been reclassified as Vibrio campbellii based on microarray comparative genome hybridization and multilocus sequence analysis. Ref
Medium 培养基 ATCC? Medium 2: Marine agar 2216 or marine broth 2216
Growth Conditions 生长条件
Temperature 培养温度: 30.0℃
Atmosphere 需氧情况: Aerobic Genotype luxM::tn5Kan
Name of Depositor 寄存人 BL
Bassler Special Collection NSF - Bacteriology
Isolation 分离源 Sensor mutant derived from Vibrio harveyi strain BB120, New Jersey, United States, 1993
References 参考文献 Bassler BL, et al. Multiple signalling systems controlling experssion of luminescence in Vibrio harveyi: Sequence and function of genes encoding a second sensory pathway. Mol. Microbiol. 13: 273-286, 1994. PubMed: 7984107
Bassler BL, et al. Cross-species induction of luminescence in the quorum-sensing bacterium Vibrio harveyi. J. Bacteriol. 179: 4043-4045, 1997. PubMed: 9190823
三、形态特征
与模式菌株Vibrio alginolyticus ATCC 17749(T) X56576相似性为99.11,与模式菌株Vibrio campbellii ATCC 25920(T) X74692相似性为99.59%;经二种特异性PCR和gyrB分子测序鉴定,定名为Vibrio alginolyticus;菌落淡黄,因长满平板而边缘不清楚,半透明;在2E平板上培养,纤维素酶阴性,甘露聚糖酶阴性,50℃不生长。嗜铁素平板28℃生长12天,产嗜铁素呈阳性(W)。
四、主要价值
主要用途为分类;研究,具体用途为病原微生物/水产经济动物重要致病菌。
五、安瓿瓶冻干管打管说明
(一)打管说明
1、安瓿瓶开封:用浸过75%酒精的脱脂棉擦净安瓿管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或者镊子敲下已破裂的安瓿管顶端。
2、菌株恢复培养:用无菌吸管吸取0.3--0.5ml适宜的液体培养基,滴入安瓿管内,轻轻振荡,使冻干菌体溶解呈悬浮状。吸取全部菌体悬浮液,移植于1-2支建议的培养基试管中,并在建议的条件下培养。
3、注意事项:菌种活化前,请将安瓿管保存在6-10℃的环境下,某些菌种经过冷冻干燥保存后,延迟期较长,需要连续两次继代培养才能正常生长。
4、复苏后的菌种在传1-2代后使用。
5、暂不启开的安瓿及复苏后需保藏的斜面应于4℃中保藏。
(二)特别注意事项
1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时问过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染;
3、斜面菌种保藏时间通常为1-2个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为5~10年;
4、菌种使用过程中如出现杂菌污染或菌种生产性能下降,应及时与微生物菌种查询网联系或更换新的菌种。
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