Shewanella algae

平台编号：Bio-137451

 Shewanella algae Simidu et al. emend. Nozue

51181 ™

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Product category

Bacteria

Strain designation

BrY

Type strain

No

Genome sequenced strain

Yes

Isolation source

Sediment from Great Bay estuary

Geographical isolation

United States; New Hampshire

Product format

Freeze-dried

Storage conditions

2°C to 8°C

General

Specific applications

Reduces iron (ferric, FeIII) ferric iron

Resistant to chromium

Biotechnology

Preceptrol

No

Handling information

Medium

ATCC Medium 2: Marine agar 2216 or marine broth 2216

Temperature

30°C

Atmosphere

Aerobic

Handling procedure

Open vial according to enclosed instructions or visit www.atcc.org for instructions.

Rehydrate the entire pellet with approximately 0.5 mL of #2 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #2 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.

Use several drops of the primary broth tube to inoculate a #2 plate and/or #2 agar slant.

Incubate at 30°C for 24 hours.

Handling notes

To demonstrate anaerobic growth by dissimilatory iron (III) reduction, use ATCC Medium #1931 broth.

1931 Broth:  Ferric citrate Medium

Ferric citrate, 13.7 g

NaHCO3, 2.5 g

KCl, 0.1 g

NH4Cl, 1.5 g

NaH2PO4, 0.6 g

Wolfe's Vitamin Solution (see below), 10.0 mL

Wolfe's Mineral Solution (see below), 10.0 mL

Sodium lactate, 30.0 mM

Distilled water, 1.0 L

Boil ferric citrate in distilled water to dissolve; cool to room temperature.  Adjust to pH 6.6 with 10N NaOH.  Add remaining components.  Equilibrate medium under 80% N2, 20% CO2.  Dispense medium anaerobically under same gas phase.  Autoclave anaerobically at 121oC for 15 minutes.  Final pH 7.0.

Wolfe's Vitamin Solution:

Biotin, 2.0 mg

Folic acid, 2.0 mg

Pyridoxine HCl, 10.0 mg

Thiamine HCl, 5.0 mg

Riboflavin, 5.0 mg

Nicotinic acid, 5.0 mg

Calcium D-(+)-pantothenate, 5.0 mg

Vitamin B12, 0.1 mg

p-Aminobenzoic acid, 5.0 mg

Thioctic acid, 5.0 mg

Distilled water, 1.0 L

Wolfe's Mineral Solution:

Nitrilotriacetic acid, 1.5 g

MgSO4.7H2O, 3.0 g

MnSO4.H2O, 0.5 g

NaCl, 1.0 g

FeSO4.7H2O, 0.1 g

CoCl2.6H2O, 0.1 g

CaCl2, 0.1 g

ZnSO4.7H2O, 0.1 g

CuSO4.5H2O, 0.01 g

AlK(SO4)2.12H2O, 0.01 g

H3BO3, 0.01 g

Na2MoO4.2H2O, 0.01 g

Distilled water, 1.0 L

Add nitrilotriacetic acid to approximately 500 mL of water and adjust to pH 6.5 with KOH to dissolve the compound. Bring volume to 1.0 L with remaining water and add remaining compounds one at a time.

Whole-genome Sequencing

History

Deposited as

Shewanella algae Simidu et al. emend. Nozue

Depositors

F Caccavo

Type of isolate

Environmental

Cross references

GenBank AF005691 Shewanella alga DNA gyrase beta-subunit (gyrB) gene, partial cds.

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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