Kluyveromyces lactis

平台编号：Bio-105141

 Kluyveromyces lactis (Dombrowski) van der Walt  拉丁名

ATCC 8585 编号

Strain Designations 菌种别名 [61, type C, CBS 2359, CCRC 21716, DSM 70799, IFO 1267, NRRL Y-1140, VKPM Y-1174]

Application 用途

Degrades whey permeate

Hydrolyzes whey lactose

Produces L-methionine

Produces beta-galactosidase beta-D-galactosidase

Produces killer toxin

Produces L-methionine from whey permeate

Biosafety Level 安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Storage Conditions 培养条件

Frozen 冷冻物 : -80°C or colder

Freeze-Dried 冻干物 : 2°C to 8°C

Live Culture: See Propagation Section

Type Strain 模式菌株 no

Genotype a [k1+ k2+]

Preceptrol no

Genome Sequenced Strain 

Yes

Mating Type Mates with ATCC 8651

Ploidy haploid

Comments 注释 Regulation of pyruvate dehydrogenase activity by E1alpha subunit levels

Resistant to killer toxin produced by Schwanniomyces occidentalis (ATCC 44252)

Carries two linear DNA plasmids, pGK11 and pGK12

Transcriptional regulation of beta-galactosidase gene

Effect of lactose permeabilization on beta-galactosidase production

Nonflocculating

Killer, immune

Genome sequencing strain (Genolevures Consortium, France).

Sequenced mitochondrial genome.

Medium 培养基 ATCC Medium 200: YM agar or YM broth

ATCC Medium 28: Emmons' modification of Sabouraud's agar

ATCC Medium 1245: YEPD

Growth Conditions 生长条件 

Temperature 培养温度 : 24°C to 26°C

Atmosphere 需氧情况 : Typical aerobic

Sequenced Data 

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

AAGGATCATTAAAGATTATGAATGAGTAGATTACTGGGGGAATCGCTGAATATGGCCTGCGCTTAATTGCGCGGCTAATTCTTGATTTTCTGCTATCAGTTTTCTTTCTCTCATCCTAAACACAATGGAGTTTTTTCTCTATGAACTACTTCCCTGGAGAGCTCGTCTCTCCAGTGGACATAAACACAAACAACATTTTGCATTATGAAAAACTATTTATCAAGAAATTTAATATTCAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAATTGCGATATGTATTGTGAATTGCAGATTTTCGTGAATCATCAAATCTTTGAACGCACATTGCGCCCTCTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCTTTGGGTTTGGTAGTGAGTGATACTCGTTTTTCGGGTTAACTTGAAAGTGGCTAGCCGTTGCCTTCTGCGTGAGCAGGGCTGCGTGTCAAGTCTATGGACTCGACTCTTGCACATCTACGTCTTAGGTTTGCGCCAATTCGTGGTAAGCTAGGGTCAATGAGCTTATAGGTGTTATAAAGACTCGCTGGTGTTTGTCTCCTTGAGGCATACGGCTTAATCAAAACTCTCAAAGTTTGACCTCAAATCAGGTAGGAATACCCGCTGAACTTAAG

D1D2 region of the 26S ribosomal RNA gene

CATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGCGTCTTCGACGTCCGAGTTGTAATTTGAAGAAGGCAACTTTGTAGCTGGTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGATCCCAGTTATTTGTAAAGTGCTTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGCGTTTGCTTCGGCTTTCGCTGGGCCAGCATCAGTTTTAGCGGTTGGATAAATCCTCGGGAATGTGGCTCTGCTTCGGTAGAGTGTTATAGCCCGTGGGAATACAGCCAGCTGGGACTGAGGATTGCGACTTTTGTCAAGGATGCTGGCGTAATGGTTAAATGCCG

Name of Depositor 寄存者 Lederle Labs.

Chain of Custody 来源国家 ATCC <-- Lederle Labs. <-- N.C. Laffer 61, type C (Zygosaccharomyces lactis)

Isolation 分离基物 Creamery, USA

Cross References 

Nucleotide (GenBank) : KU729077 ITS including 5.8S rRNA gene

Nucleotide (GenBank) : KU729173 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : X72499 nucleotide sequence of KlGGS1 gene

Nucleotide (GenBank) : X81421 K.lactis gene for alpha-alpha-trehalose 1-D-glucohydrolase.

Nucleotide (GenBank) : CR382121 Kluyveromyces lactis strain NRRL Y-1140 chromosome A complete sequence.

References 参考文献

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Lacy LR, Dickson RC. Transcriptional regulation of the Kluyveromyces lactis beta- galactosidase gene. Mol. Cell. Biol. 1: 629-634, 1981. PubMed: 9279376

. . Biotechnol. Tech. 6: 289-292, 1992.

Schaffrath R, Meacock PA. Kluyveromyces lactis killer plasmid pGKL2: molecular analysis of an essential gene, ORF5. Yeast 11: 615-628, 1995. PubMed: 7483835

Bellal M, et al. Structural differences in parietal peptidomannans from flocculating strains of Kluyveromyces lactis. Can. J. Microbiol. 41: 323-335, 1995.

Luyten K, et al. Disruption of the Kluyveromyces lactis GGS1 gene causes inability to grow on glucose and fructose and is suppressed by mutations that reduce sugar uptake. Eur. J. Biochem. 217: 701-713, 1993. PubMed: 8223613

Dickson RC, Markin JS. Physiological studies of beta-galactosidase induction in Kluyveromyces lactis. J. Bacteriol. 142: 777-785, 1980. PubMed: 6769910

Siso MI. Beta-galactosidase production by Kluyveromyces lactis on milk whey: batch versus fed-batch cultures. Process Biochem. 29: 565-568, 1994.

Bellal M, et al. Flocculation dispersion in Kluyveromyces lactis. Process Biochem. 30: 641-648, 1995.

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Butler AR, et al. Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757, 1991. PubMed: 1955863

Zeeman AM, et al. Regulation of pyruvate metabolism in chemostat cultures of Kluyveromyces lactis CBS 2359. Yeast 16: 611-620, 2000. PubMed: 10806423

Chen WB, et al. Isolation, purification, and characterization of a killer protein from Schwanniomyces occidentalis. Appl. Environ. Microbiol. 66: 5348-5352, 2000. PubMed: 11097913

Kitamoto HK, Nakahara T. Isolation of an L-methionine-enriched mutant of Kluyveromyces lactis grown on whey permeate. Process Biochem. 29: 127-131, 1994.

Dujon B, et al. Genome evolution in yeasts. Nature 430: 35-44, 2004.

Souciet JL, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 1. A set of yeast species for molecular evolution studies. FEBS Lett. 487: 3-12, 2000.

Bolotin-Fukuhara M, et al. Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis. FEBS Lett. 487: 66-70, 2000.

Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262

Zivanovic Y, et al. Complete nucleotide sequence of the mitochondrial DNA from Kluyveromyces lactis. FEMS Yeast Res. 5: 315-322, 2005. PubMed: 15691736