Aspergillus brasiliensis

平台编号：Bio-104500

 Aspergillus brasiliensis Varga et al. 拉丁名

ATCC CRM-16404 编号

Deposited As Aspergillus niger van Tieghem, anamorph

Strain Designations 菌种别名 WLRI 034(120) [CBS 733.88, DSM 1387, DSM 1988, IFO 9455, IMI 149007, NCPF 2275]

Application 用途

For use in testing and calibration in ISO 17025 accredited laboratories, to challenge assay performance, validate or compare test methods, and to establish sensitivity, linearity and specificity during assay validation or implementation. 

assay of antimicrobial preservatives

media testing

preparatory test control

quality control strain

sterility testing

testing

testing fungicides

transformation host

validation control

Antimicrobial effectiveness testing Ref

Microbial enumeration testing Ref

This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method E979-91 where only the taxon is specified.

Biosafety Level 安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 freeze-dried

Type Strain 模式菌株 no

Intended Use 

Certified Reference Material produced under an ISO 17034 accredited process.

Preceptrol? no

Comments Certificates of Analysis are available electronically at www.atcc.org, or by hardcopy upon request.

Certified Reference Material produced under an ISO 17034 accredited process.

This strain was recently re-named as Aspergillus brasiliensis according to the reference Varga et al. 2007. 

Use of impedance for preservative efficacy testing

Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.

Medium 培养基 ATCC? Medium 336: Potato dextrose agar (PDA)

ATCC? Medium 325: Malt extract agar (Blakeslee's formula)

ATCC? Medium 28: Emmons' modification of Sabouraud's agar

Growth Conditions 生长条件

Temperature 培养温度 : 24°C to 30°C

Atmosphere 需氧情况 : Typical aerobic

Morphology Colonies initially white or yellowish, mycelium growing rapidly (to cover plate in 5 to 10 days), soon producing dense layer of erect smooth-stiped, thick-walled conidiophores terminated by globose vesicles bearing phialides (uniseriate) or (commonly) metulae with phialides (biseriate) which produce dry chains of conidia. Revers of plate pale to grayish or greenish yellow, often showing radiating ridges in mycelium. Spore heads radiate, sometimes dividing into columns with age, initially pale, becoming dark brown to black. Individual conidia spherical, mid-to-dark brown, highly roughened with ridges and blunt or pointed protuberances, (3-)4-5(-6) micrometers in diameter.

Name of Depositor 寄存者 SM Ringel

Isolation 分离基物 Blueberry, North Carolina, USA

Cross References 

Nucleotide (GenBank) : KU729174 D1/D2 region of 28S rRNA gene

Nucleotide (GenBank) : FJ195348 ITS including 5.8S rRNA gene

References 参考文献

Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407

Wnendt S, et al. Molecular cloning, sequence analysis and expression of the gene encoding an antifungal-protein from Aspergillus giganteus. Curr. Genet. 25: 519-523, 1994. PubMed: 8082203

ASTM International Standard Test Method for Preservatives in Water-Containing Cosmetics. West Conshohocken, PA

British Pharmacopoeia Commission Test for sterility. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI A, 2003

British Pharmacopoeia Commission Tests for microbial contamination. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI B, 2003

British Pharmacopoeia Commission Efficacy of antimicrobial preservation. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVI C, 2003

Wnendt S, et al. Characterization of the gene encoding alpha-sarcin, a ribosome- inactivating protein secreted by Aspergillus giganteus. Gene 124: 239-244, 1993. PubMed: 8444347

Shen LL, et al. DNA topoisomerases from pathogenic fungi: targets for the discovery of antifungal drugs. Antimicrob. Agents Chemother. 36: 2778-2784, 1992. PubMed: 1336349

European Pharmacopoeia Commission Efficacy of antimicrobial preservation. Strasbourg, France:European Pharmacopoeia Commission;European Pharmacopoeia EP 5.1.3, 1997

U.S. Pharmacopeia General Chapters:<51> ANTIMICROBIAL EFFECTIVENESS TESTING. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

U.S. Pharmacopeia General Chapters: <71> STERILITY TESTS. Rockville, MD:U.S. Pharmacopeia;USP USP34-NF29, 2011

U.S. Pharmacopeia Dietary Supplement Chapters: <2021> Microbial Enumeration Tests-Nutritional and Dietary Supplements. Rockville, MD: U.S. Pharmacopeia; USP USP34-NF29, 2011

Moreira MT, et alEffect of pulsation on morphology of Aspergillus niger and Phanerochaete chrysosporium in a fluidized-bed reactorIn: Moreira MT, et alImmobilized cells: basics and applicationsAmsterdamElsevierpp. 518-523, 1996

Capobianco JO, et al. Cellular accumulation, localization, and activity of a synthetic cyclopeptamine in fungi. Antimicrob. Agents Chemother. 42: 389-393, 1998. PubMed: 9527791

FF MicroPlate. Biolog.

Efficacy of Preservation on Non-Eye Area Water-Miscible Cosmetic and Toiletry Formaulations. Gaithersburg, MD:AOAC International;AOAC "Official Methods of Analysis of the AOAC International" 998.10.

Sterility test, Growth promotion test. Tokyo, Japan:Japanese Pharmacopoeia;JP JP14e.part I.54.