供货周期: | -- |
品牌: | 百欧博伟生物 |
规格: | 冻干粉 |
货号: | bio-104064 |
CAS号: | ATCC 200808 |
平台编号:bio-104064
Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名
ATCC 200808 编号
Deposited As Saccharomyces cerevisiae Hansen
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations 菌株别名 YM4271
Alternate State Candida robusta Diddens et Lodder
Application
transformation host
transformation host for selecting DNA binding sites with colony sectoring assay
Biosafety Level 安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions 培养条件
Frozen 冷冻物 : -80°C or colder
Freeze-Dried 冻干物 : 2°C to 8°C
Live Culture : See Propagation Section
Type Strain 模式菌株 no
Genotype MATa ura3-52 his3-delta200 ade2-101 lys2-801 leu2-3 leu2-112 trp1-901 gal4-delta512 gal80-delta538 ade5::hisG
Preceptrol no
Mating Type a
Ploidy haploid
Comments
Note: This material is available under the conditions that you will not use it for commercial purposes or distributed it to third parties.
Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.
Medium ATCC Medium 1245: YEPD
ATCC 培养基 Medium 1069: YPAD medium
Growth Conditions 生长条件
Temperature 培养温度 : 24°C to 26°C
Atmosphere: Typical aerobicSequenced Data
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGGGCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTTTGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 28S ribosomal RNA gene
ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC
Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.
Name of Depositor M Johnston
Special Collection NCRR Contract
Chain of Custody 来源国家 ATCC <-- M Johnston <-- J.D. Liu
References 参考文献
Liu J, et al. Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods 5: 125-137, 1993.
Luo Y, et al. Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems. BioTechniques 20: 564-568, 1996. PubMed: 8800670
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