Saccharomyces cerevisiae Meyen ex E.C. Hansen

平台编号：bio-104064

Saccharomyces cerevisiae Meyen ex E.C. Hansen 拉丁名

ATCC 200808 编号

Deposited As Saccharomyces cerevisiae Hansen

Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Strain Designations 菌株别名 YM4271

Alternate State Candida robusta Diddens et Lodder

Application

transformation host

transformation host for selecting DNA binding sites with colony sectoring assay

Biosafety Level 安全等级 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format 提供形式 frozen

Storage Conditions 培养条件

Frozen 冷冻物 : -80°C or colder

Freeze-Dried 冻干物 : 2°C to 8°C

Live Culture  : See Propagation Section

Type Strain 模式菌株 no

Genotype MATa ura3-52 his3-delta200 ade2-101 lys2-801 leu2-3 leu2-112 trp1-901 gal4-delta512 gal80-delta538 ade5::hisG

Preceptrol no

Mating Type a

Ploidy haploid

Comments

Note: This material is available under the conditions that you will not use it for commercial purposes or distributed it to third parties.

Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.

Medium ATCC Medium 1245: YEPD

ATCC 培养基 Medium 1069: YPAD medium

Growth Conditions 生长条件 

Temperature 培养温度 : 24°C to 26°C

Atmosphere: Typical aerobicSequenced Data

18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence

GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTAAAGAAATTTAATAATTTTGAAAATGGATTTTTTTGTTTTGGCAAGAGCATGAGAGCTTTTACTGGGCAAGAAGACAAGAGATGGAGAGTCCAGCCGGGCCTGCGCTTAAGTGCGCGGTCTTGCTAGGCTTGTAAGTTTCTTTCTTGCTATTCCAAACGGTGAGAGATTTCTGTGCTTTTGTTATAGGACAATTAAAACCGTTTCAATACAACACACTGTGGAGTTTTCATATCTTTGCAACTTTTTCTTTGGGCATTCGAGCAATCGGGGCCCAGAGGTAACAAACACAAACAATTTTATCTATTCATTAAATTTTTGTCAAAAACAAGAATTTTCGTAACTGGAAATTTTAAAATATTAAAAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCCTTCTCAAACATTCTGTTTGGTAGTGAGTGATACTCTTTGGAGTTAACTTGAAATTGCTGGCCTTTTCATTGGATGTTTTTTTTCCAAAGAGAGGTTTCTCTGCGTGCTTGAGGTATAATGCAAGTACGGTCGTTTTAGGTTTTACCAACTGCGGCTAATCTTTTTTTATACTGAGCGTATTGGAACGTTATCGATAAGAAGAGAGCGTCTAGGCGAACAATGTTCTTAAAGTTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA

D1D2 region of the 28S ribosomal RNA gene

ATATCAATAAGCGGAGGAAAAGAAACCAACCGGGATTGCCTTAGTAACGGCGAGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTACCTTCGGTGCCCGAGTTGTAATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCTTTGTAAAGTGCCTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAGCGAACAAGTACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGCATTTGATCAGACATGGTGTTTTGTGCCCTCTGCTCCTTGTGGGTAGGGGAATCTCGCATTTCACTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGAATGTAGCTTGCCTCGGTAAGTATTATAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGCGACGTAAGTCAAGGATGCTGGCATAATGGTTATATGCCGC

Morphology On YPAD medium at 25°C after 7 days, colonies white to buff, smooth, butyrous, margin entire becoming slightly eroded. Cells hyaline, subglobose to broadly ellipsoidal, smooth, 6-7.5 X 4.5-6 ?m. Pseudohyphae not observed.

Name of Depositor M Johnston

Special Collection NCRR Contract

Chain of Custody 来源国家 ATCC <-- M Johnston <-- J.D. Liu

References 参考文献

Liu J, et al. Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods 5: 125-137, 1993.

Luo Y, et al. Cloning and analysis of DNA-binding proteins by yeast one-hybrid and one-two-hybrid systems. BioTechniques 20: 564-568, 1996. PubMed: 8800670