供货周期: | 一周 |
品牌: | 博尔森 |
规格: | 20μg |
货号: | BES20023AP |
CAS号: |
Neuropilin-1 Protein, Human, Recombinant (His Tag)
别称:BDCA4 Protein, Human; CD304 Protein, Human; Neuropilin-1 Protein, Human; NP1 Protein, Human; NRP Protein, Human; VEGF165R Protein, Human
纯度:> 95 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:1. Using the Octet RED System, the affinity constant (Kd) of NRP1-his bound to biotinylated human VEGF165 was 25nM.
2. Measured by its binding ability in a functional ELISA. Immobilized NRP1-His (Cat:10011-H08H) at 10 μg/mL (100 μL/well) can bind VEGF165/Biotin (Cat:11066-HNAH), the EC50 of VEGF165/Biotin (Cat:11066-HNAH) is 18-40 ng/mL.
蛋白构建:A DNA sequence encoding the human NRP1 isoform b (NP_001019799.1) (Met 1-Lys 644) was fused with a polyhistidine tag at the C-terminus.
NP No.:NP_001019799.1
表达宿主:HEK293 Cells
种属:Human
预测 N 端:Phe 22
分子量:The secreted recombinant human NRP1 consists of 634 amino acids and has a calculated molecular mass of 71.3 kDa. As a result of glycosylation, rh NRP1 migrates as an approximately 88 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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