供货周期: | 一周 |
品牌: | 博尔森 |
规格: | 5μg |
货号: | BES20219AP |
CAS号: |
HGF Protein, Human, Recombinant
纯度:> 93 % as determined by SDS-PAGE
内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
生物活性:Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 0.5-5 ng/mL.
蛋白构建:A DNA sequence encoding the human HGF (NP_000592.3) precursor (Met 1-Ser 728) was expressed and purified.
NP No.:NP_000592.3
表达宿主:CHO Stable Cells
种属:Human
预测 N 端:Gln 32 (α chain) & Val 495 (β chain)
分子量:The secreted recombinant human HGF consists of 697 amino acids after cleavage of the signal peptide and has a predicted molecular mass of 79.7 kDa. The HGF single chain can be processed into the active form of disulfide-linked heterodimer of α and β chain. As a result of glycosylation, it migrates with the apparent molecular mass of 90, 60 and 34 kDa corresponding to the single chain, α chain and β chain respectively in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研,不能用于临床诊断!所有产品仅供科研使用,不得用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务。
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