产品详情
EPOR Protein, Mouse, Recombinant (hFc Tag): 产品信息
纯度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.05-0.2 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized mouse EPO-His (Cat:51099-M08H) at 10μg/mL (100 μL/well) can bind mouse EPOR-Fc. The EC50 of mouse EPOR-Fc is 0.06-0.13μg/mL.
蛋白构建
A DNA sequence encoding the mouse EPOR (NP_034279.3) (Met1-Pro249) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
表达宿主
Baculovirus-Insect Cells
分子量
The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.
缓冲液
Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。
EPOR Protein, Mouse, Recombinant (hFc Tag)信息由上海博尔森生物科技有限公司为您提供，如您想了解更多关于EPOR Protein, Mouse, Recombinant (hFc Tag)报价、型号、参数等信息， 欢迎来电或留言咨询。除供应EPOR Protein, Mouse, Recombinant (hFc Tag)外，上海博尔森生物科技有限公司还可为您提供Carboxypeptidase B1蛋白，人类，重组（His 标签）、SCF蛋白，人类，重组（aa 1-189, His 标签）等产品， 公司有专业的客户服务团队，是您值得信赖的合作伙伴。
