产品详情
CNTF Protein, Rat, Recombinant: 产品信息
纯度
> 92 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized rat CNTF (Cat: 80012-RNAE) at 10 μg/ml (100 μl/well) can bind biotinylated rat CNTFR-His (aa 1-346) (Cat: 80019-R08B) with a linear range of 1.56-50.0 ng/ml.
2. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 30-120 ng/ml.
蛋白构建
A DNA sequence encoding the rat CNTF (P20294) (Met 1-Met 200) was expressed and purified.
分子量
The recombinant rat CNTF consisting of 200 amino acids and has a calculated molecular mass of 22.9 kDa. It migrates as an approximately 24 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile 50mM Tris, 0.1M NaCl, pH 7.5
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研,不能用于临床诊断!所有产品仅供科研使用,不得用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务。
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