产品详情
IL5RA Protein, Human, Recombinant (His Tag): 产品信息
纯度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its binding ability in a functional ELISA.
2. Immobilized recombinant mouse IL5-his at 5 μg/mL (100 μL/well) can bind biotinylated human IL5Ra-his with a linear range of 32-4000 ng/mL.
3. Measured by its ability to inhibit IL5 dependent proliferation of TF1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-2.8 μg/mL.
蛋白构建
A DNA sequence encoding the human IL5Ra isoform 2 (NP_783852.1) (Met 1-Arg 335) was expressed, fused with a polyhistidine tag at the C-terminus.
分子量
The recombinant human IL5Ra consists of 326 amino acids after removal of the signal peptide and has a predicted molecular mass of 37.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhIL5Ra is approximately 45-50 kDa due to glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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