产品详情
PTP alpha/PTPRA Protein, Human, Recombinant (aa 174-793, His & GST Tag): 产品信息
纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its ability to bind biotinylated recombinant mouse SRC in a functional ELISA.
2. Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >40000 pmol/min/μg.
蛋白构建
A DNA sequence encoding the human PTPRA isoform 2 (P18433-2) cytoplasmic domain (Ala 174-Lys 793) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主
Baculovirus-Insect Cells
分子量
The recombinant human PTPRA (aa 174-793)/GST chimera consists of 857 amino acids and has a calculated molecular mass of 99 kDa. It migrates as an approximately 90 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 20% gly, 3mM DTT
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
Liquid. It is shipped out with blue ice.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研,不能用于临床诊断!所有产品仅供科研使用,不得用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务。
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