产品详情
NKG2D Protein, Rhesus, Recombinant (aa 78-216, His Tag): 产品信息
纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human ULBP1-Fch (Cat:10679-H03H), The EC50 of human ULBP1-Fch (Cat:10679-H03H) is 0.12-0.28 μg/ml.
2. Immobilized Rhesus His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch (Cat:10759-H03H), The EC50 of human MICB-Fch (Cat:10759-H03H) is 33.8-79 ng/ml.
蛋白构建
A DNA sequence encoding the rhesus NKG2D (NP_001028061.1) (Phe78-Val216) was expressed with a polyhistide tag at the N-terminus.
表达宿主
Baculovirus-Insect Cells
分子量
The recombinant rhesus NKG2D consists of 155 amino acids and has a calculated molecular mass of 18.3 kDa.
缓冲液
Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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