产品详情
Fas/CD95 Protein, Rat, Recombinant (His Tag): 产品信息
纯度
> 90 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells.
The ED50 for this effect is typically 2-10 μg/mL in the presence of 20 ng/mL recombinant human Fas ligand.
蛋白构建
A DNA sequence encoding the rat FAS (NP_631933.2) extracellular domain (Met 1-Lys 170) was fused with a polyhistidine tag at the C-terminus.
分子量
The recombinant rat FAS comprises 160 amino acids and predicts a molecular mass of 18 kDa. As a result of glycosylation, the apparent molecular mass of the rat FAS is approximately 30-35 kDa in SDS-PAGE under reducing conditions.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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