产品详情
IL17 Protein, Human, Recombinant: 产品信息
纯度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
1. Measured by its binding ability in a functional ELISA. Immobilized human IL17A (cat:12047-HNAS) at 10 μg/ml (100 μl/well) can bind human IL17Ra (Cat:10895-H03H), The EC50 of human IL17Ra (Cat:10895-H03H) is 20.0-48.0 ng/ml.
2. Measured by its ability to induce IL-6 secretion by NIH?3T3 mouse embryonic fibroblast cells in the presense of 20ng/mL TNFα. The ED50for this effect is 0.2-1ng/mL.
蛋白构建
A DNA sequence encoding the human IL17A (Q16552) (Met1-Ala155) was expressed.
分子量
The recombinant human IL17A consists of 132 amino acids and predicts a molecular mass of 15.1 KDa. It migrates as an approximately 16, 19, and 20 KDa band in SDS-PAGE under reducing conditions due to different glycosylation.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研，不能用于临床诊断！所有产品仅供科研使用，不得用于人或动物的治疗等任何其他用途，不为任何个人提供产品和服务。
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