产品详情
FCGRT & B2M Heterodimer Protein, Human, Recombinant (His & AVI Tag), Biotinylated: 产品信息
纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物素标记率
0.5-1 as determined by the HABA assay.
生物活性
1. Measured by its ability to bind human IgG1 (Fc) (Cat:10702-HNAC) in a functional ELISA.
2. Using the Octet RED System, the affinity constant (Kd) of FCGRT & B2M Heterodimer Protein, Human, Recombinant (AVI Tag), Biotinylated (Cat: CT071-H27H-B) bound to Herceptin was 0.03 μM.
蛋白构建
A DNA sequence encoding the extracellular domain (Met1-Ser297) of human FCGRT (NP_004098.1) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (NP_004039.1) (Met1-Met119) constructed the plasmid 2. The two plasmids were co-expressed and the human FCGRT/B2M heterodimer was purified. The purified heterodimer was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
NP No.
NP_004098.1 & NP_004039.1
分子量
The recombinant heterodimer of human FCGRT/B2M comprises 399 (300+99) amino acids and has a calculated molecular mass of 45.3 (33.6+11.7) KDa.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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