产品详情
IL-1 alpha/IL1A Protein, Mouse, Recombinant: 产品信息
纯度
> 92 % as determined by SDS-PAGE
内毒素
Please contact us for more information.
生物活性
1. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.
2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.
The ED50 for this effect is typically 15-60 ng/mL.
蛋白构建
A DNA sequence encoding the mature form of mouse IL1A (NP_034684.2) (Ser 115-Ser 270) was expressed, with an initial Met at the C-terminus.
分子量
The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted.
缓冲液
Lyophilized from sterile PBS, pH 7.4
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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