产品详情
TNFR-2 / CD120b Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag): 产品信息
纯度
> 95 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to inhibit TNFα-mediated cytotoxicity in L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 7-32 ng/mL in the presence of 1 ng/mL of cynoTNFα.
蛋白构建
A DNA sequence encoding the cynomolgus / rhesus TNFRSF1B (XP_005544817.1/NP_001253134.1) (Met1-Asp257) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus TNFRSF1B sequences are identical.
NP No.
XP_005544817.1 & NP_001253134.1
分子量
The recombinant cynomolgus / rhesus TNFRSF1B is a disulfide-linked homodimer. The reduced monomer comprises 476 amino acids and has a calculated molecular mass of 52.1 KDa.The apparent molecular mass of the protein is approximately 68 KDa respectively in SDS-PAGE.
缓冲液
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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