产品详情
Acid sphingomyelinase/SMPD1 Protein, Mouse, Recombinant (His Tag): 产品信息
纯度
> 85 % as determined by SDS-PAGE
内毒素
< 1.0 EU per μg of the protein as determined by the LAL method
生物活性
Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC).
The specific activity is > 1,000 pmoles/min/μg.
蛋白构建
A DNA sequence encoding the mouse SMPD1 (Q04519) (Met 1-Leu 626) was expressed,with a C-terminal polyhistidine tag.
表达宿主
Baculovirus-Insect Cells
分子量
The secreted recombinant mouse SMPD1 consists of 592 amino acids and has a calculated molecular mass of 66.3 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.
缓冲液
Supplied as sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20
Please contact us for any concerns or special requirements.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式
Liquid. It is shipped out with blue ice.
稳定性 & 储存条件
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
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