供货周期: | 一周 |
品牌: | 博尔森 |
规格: | 20 μg |
货号: | BES21889AP |
CAS号: |
SIRP alpha Protein, Human, Recombinant (ECD, SIRP alphaV8, mFc Tag)
纯度:> 95 % as determined by SDS-PAGE.
内毒素:< 1.0 EU per μg protein as determined by the LAL method.
生物活性:Measured by its binding ability in a functional ELISA. Immobilized human CD47-His (Cat: 12283-H08H) at 2 μg/ml (100 μl/well) can bind human SIRPa(V8)-mFc (Cat: 30015-H38H), the EC50 of human SIRPa(V8)-mFc is 2-14 ng/mL.
蛋白构建:A DNA sequence encoding the human SIRP alphaV8 (AAH38510.1, with mutation Ser 44 Leu, Ser 50 Thr, Ile 52 Thr, His 54 Arg, Val 57 Ala) (Met1-Arg369) was expressed with the Fc region of mouse IgG2a at the C-terminus.
NP No.:AAH38510.1
表达宿主:HEK293 Cells
种属:Human
预测 N 端:Glu 31
分子量:The recombinant human SIRP alphaV8 consists of 572 amino acids and predicts a molecular mass of 63.6 kDa. As a result of glycosylation, it migrates as an approximately 80.4 kDa band in SDS-PAGE under reducing conditions.
缓冲液:Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hard copy of CoA.
运输方式:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
稳定性 & 储存条件:Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
复溶:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
仅用于科研,不能用于临床诊断!所有产品仅供科研使用,不得用于人或动物的治疗等任何其他用途,不为任何个人提供产品和服务。
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